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GLP-1 ELISA Kit
Glucagon like Peptide-1 (7-36) level in serum and EDTA-plasma sample
 
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INTENDED USE

 

This high sensitive ELISA (enzyme-linked immunosorbent assay) kit is produced for the exclusively quantitative determination of glucagon-llike peptide-1 (7-36) [GLP-1 (7-36)] level in serum and EDTA-plasma sample among human, rat, mouse, goat, etc.  

 

 
   

ASSAY PRINCIPLE

 

This ELISA is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in plasma sample. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 (7-36) specific antibodies. Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidate (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of  GLP-1 (7-36) in the sample.

   
 Active GLP-1 (7-36) ELISA
 
 Measures GLP-1 (7-36) without cross reaction to:
 - GLP-1 (9-36), (7-37), (9-37), (1-36), (1-37)
 - GLP-2
 
 - Glucagon
 
 Assay Sensivity: 0.6 pmol/l
 
   
 Total GLP-1 ELISA  
 Measures the sum value of GLP-1 (7-36) and (9-36) without cross reaction to:
 - GLP-1 (7-37), (9-37), (1-36), (1-37)
 - GLP-2  
 - Glucagon  
 Assay Sensivity: 1.0 pmol/l  
   
   
RANGE OF PRODUCTS:   
 Active GLP-1 (7-36) ELISA Cat # EPI-KT871
 Total GLP-1 ELISA Cat # EPI-KT876
 GLP-1 Sample Extraction Kit Cat # EPI-KT910
   
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