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Ox-LDL ELISA
Oxidized LDL as marker for lipid peroxidation
                                                                 
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  Lipid peroxidation promotes vascular deposits

Lipid peroxidation plays an important role in the pathogenesis and progression of a number of diseases. Oxidized cholesterol, in particular oxidized Low-Density-Lipoprotein (ox-LDL), is a key player in the development of atherosclerosis and coronary heart disease.

One important aspect of pathogenesis in cardiovascular risk groups, such as patients with adipositas, metabolic syndrome, diabetes or hypertension, is an impaired microcirculation which promotes inflammatory endothelial damage of vessel walls. Subsequently, LDL-particles infiltrate from the blood stream in subendothelial areas of the vessel where oxidative stress causes lipid peroxidation of LDL by free radicals.

Oxidized LDL binds massively and in an unregulated manner to macrophages with a detrimental effect: The macrophages transform into lipid-loaded „foam cells" and activate a cascade of events marked by extensive cell proliferation and accumulation of extracellular matrix components. Cholesterol-loaded macrophage foam cells represent the earliest detectable atherosclerotic damages. These lesions ultimately represent the sites of thrombosis leading to myocardial infarction and stroke.

                                                                 
  Accurate and clinically relevant determination of oxidative stress
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The monitoring of oxidative stress biomarkers, esp. of oxidized cholesterol in cardiovascular risk groups aims at early prevention of atherosclerotic lesions and can be used for monitoring the effect of a cardiovascular therapy.

One way to determine LDL is to measure the associated transport molecule apolipoprotein B (Apo B) which reflects the number of LDL-particles. A product of lipid peroxidation of LDL by oxidative stress is malondialdehyde (MDA). The level of MDA-modified Apo B can therefore serve as benchmark for the ox-LDL status.

The basic principle of Immundiagnostik‘s ox-LDL-ELISA is the specific immunological detection of MDA-modified Apo B. This method has the advantage of measuring only oxidized lipoproteins with high sensitivity and specificity - exactly those particles, which correlate with the cardiovascular risk.

 

The exceptional clinical significance of our ELISA for atherosclerosis risk analysis has been confirmed in a clinical trial with diabetes patients (Pfützner et al., 2009). Accordingly, our assay is especially suited for monitoring cardiovascular therapies with drugs which modify LDL-particles (e.g. Pioglitazon, s. Fig.).
                                                                 
 INDICATIONS OF OX-LDL
  •  Determination of lipid peroxidation as benchmark for oxidative stress
  •  Early detection of atherosclerotic lesions
  •  Cardiovascular risk analysis in risk groups (e.g. diabetes patients)
  •  Therapy monitoring in cardiovascular diseases
                                                                 
 IMM-K7810 ox-LDL ELISA with superior sensitivity in therapy control
                                                                 
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 CITATION:

“ Based on the different epitopes of the detection antibodies, the IMM-K7810 ELISA for determination of oxLDL appeared to be more sensitive and specific to detect the modifications of the oxLDL particles that were induced by pioglitazone in our clinical study than the Mercodia assay. It may therefore be more suitable for the assessment of atherosclerotic risk change resulting from modifications in the size of oxLDL particles.“


                                                               

Literature

Pfützner A et al. (2009) Diff erences in the results and interpretation of oxidized LDL cholesterol by two ELISA assays - an evaluation with samples from the PIOstat Study. Clin Lab 55: 275-281.

Koubaa N et al. (2007) Hyperhomocysteinemia and elevated ox-LDL in Tunisian type 2 diabetic patients: Role of genetic and dietary factors. Clin Biochem 40(13-14):1007-14

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 Protocol of OX-LDL ELISA KIT (IMM-K7810)
                                                                 
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