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Neospora caninum ELISA Kit
ELISA Kit for Neospora caninum detection in serum and milk
Neospora caninum is a protozoan parasite that was originally described as a parasite in dogs, which causes myositis and encephalitis. Neosporosis beef is now recognized as a major cause of miscarriage in cattle. Neospora is responsible for 21% of miscarriages that occur in a single animal. This percentage rises to 33% for the herd as a whole. Vertical transmission is the rule (at least 80% of calves born to cows infected with HIV). The first intake of colostrum Serotesting by the calf is able to detect the infection before birth.


A recent article published by Elsevier on Spanish researchers shows that the BIO-K192 kit for Neospora caninum provides the same results if applied on both serum and milk.
"Anti-Neospora caninum antibodies in milk in relation to production losses in dairy cattle"
Marta González-Warleta, José Antonio Castro-Hermida, Carmen Carro-Corral, Mercedes Mezo
Preventive Veterinary Medicine 101 (2011) 58­ 64

A comprehensive field study was carried out with the following objectives: (a) to assess the usefulness of individual and bulk tank milk analysis for determining Neospora caninum serostatus in individual cows and herds, and (b) to study the associations between N. caninum infection status (based on milk testing), and several productive and reproductive parameters in the animals. Antibodies were detected with a commercially available ELISA test (Bio K 192/5). Analysis of paired serum and milk samples from 1134 lactating cows on 38 farms revealed that 97.6% of the ELISA results were coincident, irrespective of whether serum or milk samples were used. Moreover, multiple linear regression analysis revealed that 86.0% of the variations in ELISA values in milk were due to variations in the serum. The measurement of antibodies in bulk tank milk was a good estimator of the herd level status of N. caninum infection, and enabled detection of infection in 94.7% herds with „10.0% seropositive cows and/or in all herds with >4% highly seropositive cows. The odds ratio for abortion in seropositive animals was 9.1 times higher than in seronegative animals. The infection serostatus was also a significant risk factor, as the odds ratio for abortion was even higher (12.0 times) in cows categorized as highly seropositive. ELISA values for the bulk milk from 387 randomly selected herds were negatively associated with average milk production. Moreover, milk production losses mainly occurred on farms categorized as highly positive (i.e. herds with „20.0% seropositive cows)."

In this ELISA kit, we use a protein that is also used in our competition Neospora caninum  ELISA kit with catalogue number BIO K 218,  which currently exists only as a 2 plates kit.
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